Regulatory

Part:BBa_K4716999:Design

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


ProA constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 61


Design Notes

We use this promoter as the inducer of NorR is that we have a hypothesis that the relatively weaker constitutive promoter can rise the sensitivity of NO sensor to NO.


Source

The sequence of this gene is directly taken from the following literature's supplementary materials. Reference: Xiaoyu J. Chen et al., (2021) Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells, ACS Synthetic Biology 2021, 10 (10), 2566-2578. doi: 10.1021/acssynbio.1c00223

References